Antigen

Antigen Class

TFs and others

Antigen

Su(var)205

Cell type

Cell type Class

Cell line

Cell type

OSC

Tissue Source

ovary

Developmental Stage

adult stage

Sample

source_name

Piwi-KD OSC

cell type

Ovarian Somatic Cells (OSC)

variation

RNAi Piwi

chip antibody

anti-HP1a antibody (C1A9, DSHB)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

OSC cells were fixed, lysed, and their nuclei isolated using truChIP Chromatin Shearing Kits (Covaris) according to the manufacturer's instructions. DNA was sonicated to ~300 bases using Bioruptor (Cosmobio), then diluted with ChIP buffer. IP was performed using 3 μg of antibodies on Dynabeads-Protein G beads. Samples were reverse cross-linked, treated with RNase and Proteinase K, and DNA was extracted. DNA fragments from the ChIP experiment were sheared to ~200 bases using Covaris S220 (Covaris), and were used for library preparation with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) following the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina MiSeq

dm6

Number of total reads

3874955

Reads aligned (%)

75.8

Duplicates removed (%)

6.6

Number of peaks

1804 (qval < 1E-05)

dm3

Number of total reads

3874955

Reads aligned (%)

76.8

Duplicates removed (%)

3.9

Number of peaks

1652 (qval < 1E-05)